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Product Information
Name Human alkaline phosphatase,ALP ELISA Kit
Item No. EB1332
Cat.No. EB1332
Size 96T/48T
Speciesselect human
Storage 2-8℃
Description For research use only.
Intra-Assay CV<10%
Inter-Assay CV<12%
Expiration date 6 Months
Sample serum, plasma, culture media or any biological, plasma, culture media or any biological flu

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to  Alkaline Phosphatase (ALP). Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. 

Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for  Alkaline Phosphatase (ALP)is added to each Microelisa stripplate well and incubated. Free components are washed away. 

The TMB substrate solution is added to each well. Only those wells that contain  Alkaline Phosphatase (ALP) and HRP conjugated  Alkaline Phosphatase (ALP) antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. 

The OD value is proportional to the concentration of  Alkaline Phosphatase (ALP). You can calculate the concentration of  Alkaline Phosphatase (ALP) in the samples by comparing the OD of the samples to the standard curve.