| Product Information | |
| Name | Human anti-H1N1 influenza vaccine IgG antibody（H1N1 IgG）quantitative detection ELISA Kit |
| Item No. | EC025 |
| Principle | This ELISA kit applies the indirect technique |
| Specificity | This kit identifies natural and recombinant Human H1N1 IgG |
| Storage | 6 months at 2-8°C |
| Sensitivity | 0.5ng/m |
| Description | This kit is used for detecting the Human H1N1 IgG concentration of serum, plasma, and cell culture supernatant. |
| Intra-Assay | <15% |
| Inter-Assay | <12% |
| Sample | serum, plasma, and cell culture supernatant. |
|
Description
|
This ELISA kit applies the indirect technique. The microtiter plate has been pre-coated with Human H1N1 antigen (solid phase antigen), standards or samples are added to the microtiter plate wells, then add anti-Human IgG labeled antibody. Wells are thoroughly washed to remove all unbound components. And a sandwich complex of solid antigen-antibody enzyme-labeled secondary antibodies is formed on the solid surface of the microplate. Next, Substrate solution A and substrate solution B are added to each well, and it will produce blue materials under HRP catalysis. The enzyme-substrate reaction is terminated by the addition of sulphuric acid and the color change to yellow. Measured spectrophotometrically at a wavelength of 450 nm, the absorbance (OD value) is positively correlated with the concentration of Human H1N1 IGG in the sample. The concentration of H1N1 IGG in the samples could be determined by comparing the O.D. of the samples to the standard curve.
|
