| Product Information | |
| Name | Human coagulation factor Ⅸ（FⅨ） quantitative detection ELISA Kit |
| Item No. | EC031 |
| Principle | This ELISA kit applies the quantitative sandwich enzyme immunoassay technique. |
| Storage | 6 months at 2-8°C |
| Sensitivity | 1 ng/ml |
| Description | This kit is used for detecting the Human FⅨ concentration of serum, plasma, and cell culture supernatant. |
| Intra-Assay | <15% |
| Inter-Assay | <12% |
| Sample | serum, plasma, and cell culture supernatant. |
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Description
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This ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with Human FⅨ antibody (solid phase antigen), standards or samples are added to the microtiter plate wells, then add anti-Human FⅨ HRP labeled antibody. Wells are thoroughly washed to remove all unbound components. And a sandwich complex of solid antigen-antibody enzyme-labeled secondary antibodies is formed on the solid surface of the microplate. Next, Substrate solution A and substrate solution B are added to each well, and it will produce blue materials under HRP catalysis. The enzyme-substrate reaction is terminated by the addition of sulphuric acid and the color change to yellow. Measured spectrophotometrically at a wavelength of 450 nm, the absorbance (OD value) is positively correlated with the concentration of Human FⅨ in the sample. The concentration of FⅨ in the samples could be determined by comparing the O.D. of the samples to the standard curve.
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