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Product Information
Name Human cyclooxygenase-1,COX-1 ELISA kit
Item No. EH0556

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to COX-1. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. 

      Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for COX-1 is added to each Microelisa stripplate well and incubated. Free components are washed away. 

      The TMB substrate solution is added to each well. Only those wells that contain COX-1 and HRP conjugated COX-1 antibody will appear blue in color and then turn yellow after the addition of the stop solution. 

      The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of COX-1. You can calculate the concentration of COX-1 in the samples by comparing the OD of the samples to the standard curve.