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Product Information
Name Human Estrone (E1) ELISA Kit
Item No. EH0675
Cat.No. EH0675
Principle Sandwich
Size 96/48T
Speciesselect Human
Storage 2-8°C
Detection range 6 pg/ml -400 pg/ml
Sensitivity 1.2 pg/ml
Description For research use only.
Intra-Assay CV<10%
Inter-Assay CV<12%
Expiration date 6 Months
Sample serum, plasma, culture media or any biological, plasma, culture media or any biological flu

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to E1. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. 

Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for E1 is added to each Microelisa stripplate well and incubated. Free components are washed away. 

The TMB substrate solution is added to each well. Only those wells that contain E1 and HRP conjugated E1 antibody will appear blue in color and then turn yellow after the addition of the stop solution. 

The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of E1. You can calculate the concentration of E1 in the samples by comparing the OD of the samples to the standard curve.