Product Information | |
Name | Human High-density lipoprotein cholesterol (HDL-C) ELISA Kit |
Item No. | E10763 |
Principle | Sandwich-ELISA |
Storage | 2-8°C. |
Detection range | 26ng/ml -1500 ng/ml |
Sensitivity | 8 ng/ml |
Description | This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to HDL-C. |
Intra-Assay | CV<10% |
Inter-Assay | CV<12% |
Expiration date | 6 months |
Sample | Plasma, serum, Cell culture supernate, and other biological fluids |
Description
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This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to HDL-C. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for HDL-C is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain HDL-C and HRP conjugated HDL-C antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of HDL-C. You can calculate the concentration of HDL-C in the samples by comparing the OD of the samples to the standard curve.
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