| Product Information | |
| Name | Human Immunoglobulin G3 (IgG3) quantitative detection ELISA Kit |
| Item No. | EC029 |
| Principle | This ELISA kit applies the quantitative sandwich enzyme immunoassay technique. |
| Specificity | This kit identifies natural and recombinant Human IgG3 |
| Storage | 6 months at 2-8°C |
| Sensitivity | 1 ng/ml |
| Description | This kit is used for detecting the Human IgG3 concentration of serum, plasma, and cell culture supernatant. |
| Intra-Assay | <15% |
| Inter-Assay | <12% |
| Sample | serum, plasma, and cell culture supernatant. |
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Description
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This ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with Human IgG3 antibody (solid phase antigen), standards or samples are added to the microtiter plate wells, then add anti- Human IgG3 HRP labeled antibody. Wells are thoroughly washed to remove all unbound components. And a sandwich complex of solid antigen-antibody enzyme-labeled secondary antibodies is formed on the solid surface of the microplate. Next, Substrate solution A and substrate solution B are added to each well, and it will produce blue materials under HRP catalysis. The enzyme-substrate reaction is terminated by the addition of sulphuric acid and the color change to yellow. Measured spectrophotometrically at a wavelength of 450 nm, the absorbance (OD value) is positively correlated with the concentration of Human IgG3 in the sample. The concentration of IgG3 in the samples could be determined by comparing the O.D. of the samples to the standard curve.
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