| Product Information | |
| Name | Human Insulin Like Growth Factor 2 (IGF-2) ELISA Kit |
| Item No. | E10160 |
| Principle | Sandwich-ELISA |
| Specificity | The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to IGF-2. |
| Storage | 2-8°C |
| Detection range | 0.1 ng/ml -8 ng/ml |
| Sensitivity | 0.01 ng/ml |
| Description | This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to IGF-2. |
| Intra-Assay | CV<10% |
| Inter-Assay | CV<12% |
| Expiration date | 6 months |
| Sample | Plasma, serum, Cell culture supernate, and other biological fluids |
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Description
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This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to IGF-2. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for IGF-2 is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain IGF-2 and HRP conjugated IGF-2 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of IGF-2. You can calculate the concentration of IGF-2 in the samples by comparing the OD of the samples to the standard curve.
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