| Product Information | |
| Name | Human Interleukin 17 (IL-17) quantitative detection ELISA Kit |
| Item No. | EC022 |
| Principle | This ELISA kit applies the quantitative sandwich enzyme immunoassay technique. |
| Specificity | This kit is used for detecting the IL-17 concentration of serum, plasma, and cell culture supernatant. |
| Storage | 6 months at 2-8°C. |
| Detection range | 25 pg/mL – 800 pg/mL |
| Sensitivity | 1.0 pg/mL |
| Description | This kit is used for detecting the IL-17 concentration of serum, plasma, and cell culture supernatant. |
| Intra-Assay | <15% |
| Inter-Assay | <12% |
| Sample | serum, plasma, and cell culture supernatant. |
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Description
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This ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with Human IL-17 antibody (solid phase antigen), standards or samples are added to the microtiter plate wells, then add anti- Human IL-17 HRP labeled antibody. Wells are thoroughly washed to remove all unbound components. And a sandwich complex of solid antigen-antibody enzyme-labeled secondary antibodies is formed on the solid surface of the microplate. Next, Substrate solution A and substrate solution B are added to each well, and it will produce blue materials under HRP catalysis. The enzyme-substrate reaction is terminated by the addition of sulphuric acid and the color change to yellow. Measured spectrophotometrically at a wavelength of 450 nm, the absorbance (OD value) is positively correlated with the concentration of Human IL-17 in the sample. The concentration of IL-17 in the samples could be determined by comparing the O.D. of the samples to the standard curve.
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