| Product Information | |
| Name | Human Leptin (LEP) ELISA Kit |
| Item No. | E11303 |
| Principle | Sandwich-ELISA |
| Storage | 2-8°C. |
| Detection range | 35 pg/ml -2000pg/ml |
| Sensitivity | 12pg/ml |
| Description | This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to Leptin (LEP). |
| Intra-Assay | CV<10% |
| Inter-Assay | CV<12% |
| Expiration date | 6 months |
| Sample | Plasma, serum, Cell culture supernate, and other biological fluids |
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Description
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This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to Leptin (LEP). Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for Leptin (LEP) is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain Leptin (LEP) and HRP conjugated Leptin (LEP) antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Leptin (LEP). You can calculate the concentration of Leptin (LEP) in the samples by comparing the OD of the samples to the standard curve.
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