This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to SCD40L. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific forSCD40L is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain SCD40L and HRP-conjugated SCD40L antibodieswill appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of SCD40L. You can calculate the concentration of SCD40L in the samples by comparing the OD of the samples to the standard curve.
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Materials provided with the kit |
96 determinations |
Storage |
| 1 |
User manual |
1 |
R.T. |
| 2 |
Closure plate membrane |
2 |
R.T. |
| 3 |
Sealed bags |
1 |
R.T. |
| 4 |
Microelisa stripplate |
1 |
2-8℃ |
| 5 |
Standard：32 ng/ml |
0.5ml×1 bottle |
2-8℃ |
| 6 |
Standard diluent |
6ml×1 bottle |
2-8℃ |
| 7 |
HRP-Conjugate reagent |
10ml×1 bottle |
2-8℃ |
| 8 |
Sample diluent |
6ml×1 bottle |
2-8℃ |
| 9 |
Chromogen Solution A |
6ml×1 bottle |
2-8℃ |
| 10 |
Chromogen Solution B |
6ml×1 bottle |
2-8℃ |
| 11 |
Stop Solution |
6ml×1 bottle |
2-8℃ |
| 12 |
20 X Wash solution |
25ml×1bottle |
2-8℃ |
