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Product Information
Name VEGF (Human) ELISA Kit
Item No. E10103
Principle sandwich
Specificity This VEGF ELISA kit is intended Laboratory for Research use only and is not for use in diagnostic or therapeutic procedures.
Storage 2-8°C
Detection range 1pg/ml -400pg/ml
Sensitivity 1pg/ml
Intra-Assay <10%
Inter-Assay <12%
Expiration date 6 months
Sample Plasma, serum, Cell culture supernate, and other biological fluids
Description
This VEGF ELISA kit is intended Laboratory for Research use only and is not for use in diagnostic or therapeutic procedures. The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of VEGF in the sample, this VEGF ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus VEGF concentration. The concentration of VEGF in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 
  Materials provided with the kit 96 determinations Storage
1 User manual 1 R.T.
2 Closure plate membrane 2 R.T.
3 Sealed bags 1 R.T.
4 Microelisa stripplate 1 2-8℃
5 Standard:32 ng/ml 0.5ml×1 bottle 2-8℃
6 Standard diluent 6ml×1 bottle 2-8℃
7 HRP-Conjugate reagent 10ml×1 bottle 2-8℃
8 Sample diluent 6ml×1 bottle 2-8℃
9 Chromogen Solution A 6ml×1 bottle 2-8℃
10 Chromogen Solution B 6ml×1 bottle 2-8℃
11 Stop Solution 6ml×1 bottle 2-8℃
12 20 X Wash solution 25ml×1bottle 2-8℃