| Product Information | |
| Name | Human IL-2 ELISA KIT |
| Item No. | E10003 |
| Principle | sandwich |
| Specificity | No significant cross-reactivity or interference with humanIL-2 sR,IL-2 sR,Fc Chimera IL-2 sR,IL-2 sR and mouse IL-2, rat IL-2 |
| Storage | 2-8°C |
| Detection range | 16.5-1000 pg/ml |
| Sensitivity | 16.5pg/ml |
| Intra-Assay | <10% |
| Inter-Assay | <10% |
| Expiration date | 6 months |
| Sample | Plasma, serum, Cell culture supernate, and other biological fluids |
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Description
Materials provided with the kits
Typical Data and Standard Curve
Human IL-2 standard curve
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Introduction
Interleukin 2 (IL-2) is a pleiotropic cytokine produced primarily by T lymphocytes. The mature mouse IL-2 is a 149 amino acid (aa) residue glycoprotein. Human IL-2 is synthesized as a 153 amino acid precursor that contains a 20 amino acid signal sequence plus a 133 amino acid mature region. IL-2 plays a key role in promoting the clonal expansion of antigen-specific T cells, natural killer cells, and B-cells during certain phases of their response. Moreover, IL-2 modulates the expression of interferon-γ and major histocompatibility antigens, stimulates the proliferation and differentiation of activated B-cells, augments natural killer cell activity, and inhibits granulocyte-macrophage colony formation. In addition, IL-2 induces the proliferation and differentiation of oligodendrocytes. Principles of the Test The kit is a solid sandwich enzyme-linked immunosorbent assay for the detection of human IL-2. An anti-human IL-2 monoclonal antibody has been absorbed onto the wells of the microtiter strips provided. Samples including specimens or standards were pipetted into wells. The human IL-2 in specimens or standards would be captured by the coated antibody and the free others were removed by washing. The human IL-2 biotin-conjugated antibody was added and binds to human IL-2 captured by the first antibody, which formed a sandwich. Streptavidin-HRP would be added and binds to the biotin-conjugated antibody, then free Streptavidin-HRP would be removed during a wash step. After this, a substrate solution would be added and catalyzed by the HRP, and a colored product is formed. The intensity of the colored product is used to calculate in proportion to the amount of human IL-2 in the original specimen.
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