Product Information
Name Human IL-2 ELISA KIT
Item No. E10003
Principle sandwich
Specificity No significant cross-reactivity or interference with humanIL-2 sR,IL-2 sR,Fc Chimera IL-2 sR,IL-2 sR and mouse IL-2, rat IL-2
Storage 2-8°C
Detection range 16.5-1000 pg/ml
Sensitivity 16.5pg/ml
Intra-Assay <10%
Inter-Assay <10%
Expiration date 6 months
Sample Plasma, serum, Cell culture supernate, and other biological fluids
Materials provided with the kits
Typical Data and Standard Curve
Human IL-2 standard curve
Interleukin 2 (IL-2) is a pleiotropic cytokine produced primarily by T lymphocytes. The mature mouse IL-2 is a 149 amino acid (aa) residue glycoprotein. Human IL-2 is synthesized as a 153 amino acid precursor that contains a 20 amino acid signal sequence plus a 133 amino acid mature region. 
IL-2 plays a key role in promoting the clonal expansion of antigen-specific T cells, natural killer cells, and B-cells during certain phases of their response. Moreover, IL-2 modulates the expression of interferon-γ and major histocompatibility antigens, stimulates the proliferation and differentiation of activated B-cells, augments natural killer cell activity, and inhibits granulocyte-macrophage colony formation. In addition, IL-2 induces the proliferation and differentiation of oligodendrocytes. 

Principles of the Test
The kit is a solid sandwich enzyme-linked immunosorbent assay for the detection of human IL-2. An anti-human IL-2 monoclonal antibody has been absorbed onto the wells of the microtiter strips provided. Samples including specimens or standards were pipetted into wells. The human IL-2 in specimens or standards would be captured by the coated antibody and the free others were removed by washing. The human IL-2 biotin-conjugated antibody was added and binds to human IL-2 captured by the first antibody, which formed a sandwich. Streptavidin-HRP would be added and binds to the biotin-conjugated antibody, then free Streptavidin-HRP would be removed during a wash step. After this, a substrate solution would be added and catalyzed by the HRP, and a colored product is formed. The intensity of the colored product is used to calculate in proportion to the amount of human IL-2 in the original specimen. 

reagent 96/48Test Kit
Assay Buffer 5ml/3 ml
Human IL-2 Antibody-Coated Wells 12 strips/6 strips
Standard Diluent 10 ml/5ml
Human IL-2 Standard  2/1vial(s)
Human IL-2 Detetion Antibody 10ml/5ml
Streptavidin-HRP 10ml/5ml
Wash Buffer Concentrate 20× 30ml/15ml
TMB 10ml/5 ml
Stop Solution 5ml/3 ml
Plate Covers 3/2
Complete Instruction Manual 1
Concentration (pg/ml) Typical data 1 Typical data 2 Average 
0 0.1626 0.1378 0.1502
31.25 0.2639 0.3251 0.2945
62.5 0.3998 0.3828 0.3913
125 0.7596 0.5124 0.636
250 1.0269 0.8791 0.953
500 1.0295 1.7249 1.3772
1000 2.0145 1.9073 1.9609